BINDING OF DNA TO NATURAL SEPIOLITE: …
Sepiolite plus Mg2+ or Ca2+ is added, allowing plasmid DNA to bind to sepiolite, allowing the subse-quent washing steps to eliminate contaminants. Sepiolite-bearing DNA is centrifuged and washed with Tris buffer. EDTA is then added to the sepiolite/DNA pellets, releas-ing the plasmid DNA from the sepiolite.
